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ZytoFast CISH Probes

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Introduction


The ZytoFast® products are designed for outstandingly fast detection and discrimination of human pathogen viruses, e.g. HPV, EBV, CMV, and the determination of lymphocyte clonality by detecting Ig-κ and Ig-λ light chain RNA by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections and cell samples. The signal intensity of ZytoFast® probes is increased even more when using the  ZytoFast® PLUS Implementation Kits.


Method Description - ZytoFast®


The ZytoFast® Ig-kappa/Ig-lambda Probe uses oligonucleotide probes tagged with Digoxigenin and Biotin to determine the IGK and IGL clonality at once with a Dual Color CISH-System. Digoxigenin is detected by HRP-conjugated antibodies while Biotin is detected using AP-conjugated streptavidin. The enzymatic reaction of chromogenic substrates, e.g. HRP-Green and Permanent Red, leads to the formation of strong color precipitates that can be visualized by light microscopy.


Method Description - ZytoFast® PLUS 


The ZytoFast® PLUS system uses Digoxigenin-labeled probes which are detected using primary antibodies. These antibodies are detected by polymerized enzyme-conjugated secondary antibodies. The enzymatic reaction of chromogenic substrates, e.g. NBT/BCIP, AEC or DAB, leads to the formation of strong color precipitates that can be visualized by light microscopy.


ZytoFast® - Flexibility that meets your needs 

•All DIG-labeled probes can be combined with any of the ZytoFast® PLUS CISH Implementation Kits




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